Journal: Experimental and Therapeutic Medicine

Article Title: A rare dermatological manifestation of follicular spicules in a patient with multiple myeloma and end-stage renal disease on hemodialysis: A case report

doi: 10.3892/etm.2025.12930

Figure Lengend Snippet: SPE and immunofixation confirming monoclonal gammopathy. (A) SPE demonstrating an M-spike in the γ region. (B) Immunofixation electrophoresis revealing a monoclonal IgA λ pattern, confirming the presence of IgA-λ type multiple myeloma. (C) Immunofixation electrophoresis further confirming the monoclonal IgA λ pattern. AU, arbitrary units; SPE, serum protein electrophoresis. ELP, electrophoresis reference; G, IgG; A, IgA; M, IgM; K, κ light chain; L, λ light chain; D, IgD; E, IgE.

Article Snippet: Primary antibodies were incubated overnight at 4 ̊C, including: CD138 (cat. no. ab34164; dilution 1:100; Abcam), CD56 (cat. no. ab75813; dilution 1:200; Abcam), κ light chain (cat. no. ZM-0317; dilution 1:150; OriGene Technologies, Inc.) and λ light chain (cat. no. ZM-0318; dilution 1:150; Origene Technologies, Inc.).

Techniques: Electrophoresis, Protein Electrophoresis

Journal: Experimental and Therapeutic Medicine

Article Title: A rare dermatological manifestation of follicular spicules in a patient with multiple myeloma and end-stage renal disease on hemodialysis: A case report

doi: 10.3892/etm.2025.12930

Figure Lengend Snippet: Immunohistochemical characterization of bone marrow biopsy. (A) Congo red staining showed amyloid deposits with birefringence under polarized light, confirming amyloidosis. (B) Scattered CD3 + T cells. (C) CD20 + B cells. (D) Diffuse CD56 positivity indicating natural killer cell involvement. (E) Diffuse CD138 staining marking plasma cell infiltration. (F) Negative κ light chain staining. (G) Diffuse positive staining of λ light chains, confirming monoclonality. (H) Multiple myeloma oncogene 1-positivity indicated plasma cell differentiation. (I) Hematoxylin and eosin staining revealed diffuse infiltration by atypical lymphoid/plasma cells. (J) Positive staining for amyloid deposits in bone marrow biopsy. All images were captured under a light microscope at x400 magnification.

Article Snippet: Primary antibodies were incubated overnight at 4 ̊C, including: CD138 (cat. no. ab34164; dilution 1:100; Abcam), CD56 (cat. no. ab75813; dilution 1:200; Abcam), κ light chain (cat. no. ZM-0317; dilution 1:150; OriGene Technologies, Inc.) and λ light chain (cat. no. ZM-0318; dilution 1:150; Origene Technologies, Inc.).

Techniques: Immunohistochemical staining, Staining, Clinical Proteomics, Cell Differentiation, Light Microscopy

Journal: Experimental and Therapeutic Medicine

Article Title: A rare dermatological manifestation of follicular spicules in a patient with multiple myeloma and end-stage renal disease on hemodialysis: A case report

doi: 10.3892/etm.2025.12930

Figure Lengend Snippet: Flow cytometric immunophenotyping of bone marrow cells from a patient with PCM. (A) Lymphocyte analysis showing a decreased CD4/CD8 ratio. (B and C) Quantification of CD57⁺ LGLs, demonstrating a normal proportion of this subset. (D) Marked expansion of plasma cells detected within the bone marrow aspirate. (E) Dual-parameter plot of CD38 and CD45 expression revealing a distinct CD38 bright+ /CD45 dim+ population, accounting for 33.19% of total nucleated cells, consistent with malignant plasma cell infiltration. (F) Intracellular immunoglobulin light chain staining within the CD38 bright+ population indicating prominent λ light chain restriction (97.37%) with minimal κ expression (0.03%), confirming clonal proliferation and supporting the diagnosis of PCM. LGLs, large granular lymphocytes; NC, nucleated cell; PCM, plasma cell myeloma; A, allophycocyanin-Alexa Fluor 700; APC, allophycocyanin; CD38bri + , CD38 bright positive; DNT, double negative T cells; DPT, dual-parameter plot; ECD, energy-coupled dye; H, height parameter; KO525, Krome Orange 525; Lym, lymphocytes; NK, natural killer cells; PC5.5, phycoerythrin-cyanine 5.5; PE, phycoerythrin; SSC, side scatter.

Article Snippet: Primary antibodies were incubated overnight at 4 ̊C, including: CD138 (cat. no. ab34164; dilution 1:100; Abcam), CD56 (cat. no. ab75813; dilution 1:200; Abcam), κ light chain (cat. no. ZM-0317; dilution 1:150; OriGene Technologies, Inc.) and λ light chain (cat. no. ZM-0318; dilution 1:150; Origene Technologies, Inc.).

Techniques: Clinical Proteomics, Expressing, Staining, Biomarker Discovery